![]() Leaf senescence of tobacco is a complex process involving many molecular events along with physiological and biochemical changes, and these changes are driven by differential expression of thousands of genes under the control of highly regulated genetic procedures ( Masclaux et al., 2000 Gregersen and Holm, 2007). Leaf senescence is a complicated developmental process, which is regulated by internal genetic program and other environmental signals ( Uzelac et al., 2016). The study of tobacco leaf senescence and its internal material transport provides an important platform for understanding tobacco plant growth and development ( Gregersen et al., 2013). Common tobacco ( Nicotiana tabacum L.) is regarded as an ideal model organism to investigate leaf senescence. Leaf senescence is an important trait that affects the biomass accumulation and nutritional value of agricultural crops. Our results provided valuable information for further functional investigation of leaf senescence in plants. A total of 30 core genes were examined by Weight Gene Co-expression Network Analysis (WGCNA), and they appeared to play a crucial role in the regulatory of tobacco senescence. Furthermore, Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis found that these DEGs were enriched in pathways of carbon metabolism, starch and sucrose metabolism, nitrogen metabolism, and photosynthesis among these expression profiles. All the DEGs were grouped into 12 expression profiles according to their distinct expression patterns based on Short Time-series Expression Miner (STEM) software analysis. Gene Ontology (GO) analysis found that DEGs were enriched in biosynthetic, metabolic, photosynthesis, and redox processes, and especially, the nitrogen metabolic pathways were closely related to the whole leaf senescence process (M1∼M5). Significant changes of nitrogen, sugars, and the DEGs related to metabolite accumulation were identified, suggesting the importance of energy metabolism during leaf senescence. A total of 722, 1,534, 3,723, and 6,933 genes were differentially expressed (DEG) between M1 and M2, M1 and M3, M1 and M4, and M1 and M5, respectively. In this study, the biochemical properties and transcriptome at five maturity stages (M1∼M5) of tobacco leaves were analyzed to reveal the dynamic changes in leaf senescence of tobacco. Leaf senescence is an important process of growth and development in plant, and it is a programmed decline controlled by a series of genes. ![]() 5Nanping Tobacco Company, Nanping, China.4Jianning Branch of Sanming Tobacco Company, Sanming, China.3Yanping Branch of Nanping Tobacco Company, Nanping, China. ![]() 2Institute of Tobacco Science, Fujian Provincial Tobacco Company, Fuzhou, China.1College of Life Sciences, Fujian Agriculture and Forestry University, Fuzhou, China.Binghui Zhang 1,2 †, Jiahan Yang 1 †, Gang Gu 2, Liao Jin 3, Chengliang Chen 4, Zhiqiang Lin 3, Jiangyu Song 5 and Xiaofang Xie 1* ![]()
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